Comparison of defense responses of transgenic potato lines expressing three different Rpi genes to specific Phytophthora infestans races based on transcriptome profiling.
Identifieur interne : 000265 ( Main/Exploration ); précédent : 000264; suivant : 000266Comparison of defense responses of transgenic potato lines expressing three different Rpi genes to specific Phytophthora infestans races based on transcriptome profiling.
Auteurs : Xiaohui Yang [République populaire de Chine] ; Xiao Guo [République populaire de Chine] ; Guangxia Chen [République populaire de Chine] ; Daofeng Dong [République populaire de Chine] ; Fang Liu [République populaire de Chine] ; Yuanjun Yang [République populaire de Chine] ; Yu Yang [République populaire de Chine] ; Guangcun Li [République populaire de Chine]Source :
- PeerJ [ 2167-8359 ] ; 2020.
Abstract
Potato late blight, one of the most devastating diseases in potato, is caused by the oomycete Phytophthora infestans. Over 20 resistance genes have been cloned including R1, R3a, and R3b. The distinctions between defense response mechanisms mediated by different resistance genes are still unclear. Here we performed transcriptome profiling in three transgenic lines, R1, R3a, and R3b, and wild-type Desiree under inoculation with two P. infestans isolates, 89148 (race 0) and CN152 (super race), using RNA-seq. Compared with wild type, specific differentially expressed genes (DEGs) were identified in the three transgenic lines. The highest number of DEGs occurred in transgenic R3b, with 779 DEGs in response to isolate 89148 and 864 DEGs in response to infection by CN152, followed by transgenic R1 lines with 408 DEGs for isolate 89148 and 267 DEGs for CN152. Based on gene ontology, the most common GO terms (15 for 89148 and 20 for CN152) were enriched in transgenic R3a and R3b lines. This indicates that the defense pathways mediated by R3a and R3b are more similar than those mediated by R1. Further separate GO analysis of up- or down-regulated DEGs showed that the down-regulated DEGs mainly functioned in mediating the resistance of potato to P. infestans 89148 by response to stress biological process and to CN152 by oxidation reduction biological process. KEGG pathways of DNA replication, plant-pathogen interaction and pentose and glucuronate interconversions are unique for transgenic R1, R3a, and R3b lines in incompatible interactions. Quantitative real-time PCR experimental validation confirmed the induced expression of DEGs in the late blight resistance signaling pathway. Our results will lay a solid foundation for further understanding the mechanisms of plant-pathogen interactions, and provide a theoretical reference for durable resistance in potato.
DOI: 10.7717/peerj.9096
PubMed: 32411536
PubMed Central: PMC7207217
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Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yu" sort="Yang, Yu" uniqKey="Yang Y" first="Yu" last="Yang">Yu Yang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Li, Guangcun" sort="Li, Guangcun" uniqKey="Li G" first="Guangcun" last="Li">Guangcun Li</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32411536</idno><idno type="pmid">32411536</idno><idno type="doi">10.7717/peerj.9096</idno><idno type="pmc">PMC7207217</idno><idno type="wicri:Area/Main/Corpus">000189</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000189</idno><idno type="wicri:Area/Main/Curation">000189</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000189</idno><idno 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Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Guo, Xiao" sort="Guo, Xiao" uniqKey="Guo X" first="Xiao" last="Guo">Xiao Guo</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Guangxia" sort="Chen, Guangxia" uniqKey="Chen G" first="Guangxia" last="Chen">Guangxia Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Daofeng" sort="Dong, Daofeng" uniqKey="Dong D" first="Daofeng" last="Dong">Daofeng Dong</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Fang" sort="Liu, Fang" uniqKey="Liu F" first="Fang" last="Liu">Fang Liu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yuanjun" sort="Yang, Yuanjun" uniqKey="Yang Y" first="Yuanjun" last="Yang">Yuanjun Yang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Yu" sort="Yang, Yu" uniqKey="Yang Y" first="Yu" last="Yang">Yu Yang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Li, Guangcun" sort="Li, Guangcun" uniqKey="Li G" first="Guangcun" last="Li">Guangcun Li</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">PeerJ</title><idno type="ISSN">2167-8359</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato late blight, one of the most devastating diseases in potato, is caused by the oomycete <i>Phytophthora infestans</i>. Over 20 resistance genes have been cloned including <i>R1</i>, <i>R3a</i>, and <i>R3b</i>. The distinctions between defense response mechanisms mediated by different resistance genes are still unclear. Here we performed transcriptome profiling in three transgenic lines, <i>R1</i>, <i>R3a</i>, and <i>R3b,</i> and wild-type Desiree under inoculation with two <i>P. infestans</i> isolates, 89148 (race 0) and CN152 (super race), using RNA-seq. Compared with wild type, specific differentially expressed genes (DEGs) were identified in the three transgenic lines. The highest number of DEGs occurred in transgenic <i>R3b</i>, with 779 DEGs in response to isolate 89148 and 864 DEGs in response to infection by CN152, followed by transgenic <i>R1</i> lines with 408 DEGs for isolate 89148 and 267 DEGs for CN152. Based on gene ontology, the most common GO terms (15 for 89148 and 20 for CN152) were enriched in transgenic <i>R3a</i> and <i>R3b</i> lines. This indicates that the defense pathways mediated by <i>R3a</i> and <i>R3b</i> are more similar than those mediated by <i>R1</i>. Further separate GO analysis of up- or down-regulated DEGs showed that the down-regulated DEGs mainly functioned in mediating the resistance of potato to <i>P. infestans</i> 89148 by response to stress biological process and to CN152 by oxidation reduction biological process. KEGG pathways of DNA replication, plant-pathogen interaction and pentose and glucuronate interconversions are unique for transgenic <i>R1</i>, <i>R3a</i>, and <i>R3b</i> lines in incompatible interactions. Quantitative real-time PCR experimental validation confirmed the induced expression of DEGs in the late blight resistance signaling pathway. Our results will lay a solid foundation for further understanding the mechanisms of plant-pathogen interactions, and provide a theoretical reference for durable resistance in potato.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32411536</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2167-8359</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>PeerJ</Title><ISOAbbreviation>PeerJ</ISOAbbreviation></Journal><ArticleTitle>Comparison of defense responses of transgenic potato lines expressing three different <i>Rpi</i> genes to specific <i>Phytophthora infestans</i> races based on transcriptome profiling.</ArticleTitle><Pagination><MedlinePgn>e9096</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.7717/peerj.9096</ELocationID><Abstract><AbstractText>Potato late blight, one of the most devastating diseases in potato, is caused by the oomycete <i>Phytophthora infestans</i>. Over 20 resistance genes have been cloned including <i>R1</i>, <i>R3a</i>, and <i>R3b</i>. The distinctions between defense response mechanisms mediated by different resistance genes are still unclear. Here we performed transcriptome profiling in three transgenic lines, <i>R1</i>, <i>R3a</i>, and <i>R3b,</i> and wild-type Desiree under inoculation with two <i>P. infestans</i> isolates, 89148 (race 0) and CN152 (super race), using RNA-seq. Compared with wild type, specific differentially expressed genes (DEGs) were identified in the three transgenic lines. The highest number of DEGs occurred in transgenic <i>R3b</i>, with 779 DEGs in response to isolate 89148 and 864 DEGs in response to infection by CN152, followed by transgenic <i>R1</i> lines with 408 DEGs for isolate 89148 and 267 DEGs for CN152. Based on gene ontology, the most common GO terms (15 for 89148 and 20 for CN152) were enriched in transgenic <i>R3a</i> and <i>R3b</i> lines. This indicates that the defense pathways mediated by <i>R3a</i> and <i>R3b</i> are more similar than those mediated by <i>R1</i>. Further separate GO analysis of up- or down-regulated DEGs showed that the down-regulated DEGs mainly functioned in mediating the resistance of potato to <i>P. infestans</i> 89148 by response to stress biological process and to CN152 by oxidation reduction biological process. KEGG pathways of DNA replication, plant-pathogen interaction and pentose and glucuronate interconversions are unique for transgenic <i>R1</i>, <i>R3a</i>, and <i>R3b</i> lines in incompatible interactions. Quantitative real-time PCR experimental validation confirmed the induced expression of DEGs in the late blight resistance signaling pathway. Our results will lay a solid foundation for further understanding the mechanisms of plant-pathogen interactions, and provide a theoretical reference for durable resistance in potato.</AbstractText><CopyrightInformation>©2020 Yang et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y" EqualContrib="Y"><LastName>Yang</LastName><ForeName>Xiaohui</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y" EqualContrib="Y"><LastName>Guo</LastName><ForeName>Xiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Guangxia</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Daofeng</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Fang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yuanjun</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Shandong Academy of Agricultural Sciences, Molecular Biology Key Laboratory of Shandong Facility Vegetable/National Vegetable Improvement Center Shandong Subcenter/ Huang-Huai-Hai Region Scientific Observation and Experimental Station of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Jinan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Guangcun</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences; Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture and Rural Affairs, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>05</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PeerJ</MedlineTA><NlmUniqueID>101603425</NlmUniqueID><ISSNLinking>2167-8359</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Late blight</Keyword><Keyword MajorTopicYN="N">Mechanisms</Keyword><Keyword MajorTopicYN="N">Potato</Keyword><Keyword MajorTopicYN="N">Resistance</Keyword></KeywordList><CoiStatement>The authors declare there are no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>10</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>04</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>5</Month><Day>16</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32411536</ArticleId><ArticleId 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Li</name><name sortKey="Liu, Fang" sort="Liu, Fang" uniqKey="Liu F" first="Fang" last="Liu">Fang Liu</name><name sortKey="Yang, Yu" sort="Yang, Yu" uniqKey="Yang Y" first="Yu" last="Yang">Yu Yang</name><name sortKey="Yang, Yuanjun" sort="Yang, Yuanjun" uniqKey="Yang Y" first="Yuanjun" last="Yang">Yuanjun Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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